International immunology, 2002
Department of Neurobiology and Anatomy
Drexel University College of Medicine
2900 W. Queen Lane, Room186
Park, C., Yang, Y.-fu, Zhou, X., Toyooka, K., Yashiro‐Ohtani, Y., Park, W., … Fujiwara, H. (2002). Reversible CD8 expression induced by common cytokine receptor gamma chain-dependent cytokines in a cloned CD4(+) T(h)1 cell line. International Immunology.
Park, C., Yi-fu Yang, Xuyu Zhou, K. Toyooka, Y. Yashiro‐Ohtani, W. Park, M. Tomura, X. Tai, T. Hamaoka, and H. Fujiwara. “Reversible CD8 Expression Induced by Common Cytokine Receptor Gamma Chain-Dependent Cytokines in a Cloned CD4(+) T(h)1 Cell Line.” International immunology (2002).
Park, C., et al. “Reversible CD8 Expression Induced by Common Cytokine Receptor Gamma Chain-Dependent Cytokines in a Cloned CD4(+) T(h)1 Cell Line.” International Immunology, 2002.
T cells that are intrathymically lineage committed are believed to maintain their CD4 or CD8 co-receptor expression. Here, we investigated whether intrathymic lineage commitment involves irreversible genetic modification or whether co-receptor expression can be reprogrammed depending on external stimuli. The CD4(+) T(h)1 clone 2D6 established from splenic T cells as an IL-12-dependent line survived in culture with IL-2, IL-7 or IL-15 alone. Surprisingly, CD8 expression occurred in 2D6 cells upon replacement of IL-12 with any one of the three cytokines that stimulate the common cytokine receptor gamma chain, yielding CD4(+)CD8(+) 2D6 cells. CD8 expression declined when IL-2 was replaced with IL-12 and CD8 induction was inhibited when IL-12 was included in IL-2 or IL-7 culture. Our observations show that even a lineage-committed mature T cell can be reprogrammed for co-receptor expression in response to particular external stimuli.